Physiological and proteomics analyses of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) responses to Phytophthora cinnamomi.
Identifieur interne : 001251 ( Main/Exploration ); précédent : 001250; suivant : 001252Physiological and proteomics analyses of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) responses to Phytophthora cinnamomi.
Auteurs : Besma Sghaier-Hammami [Tunisie] ; José Valero-Galvàn ; M Cristina Romero-Rodríguez ; Rafael Ma Navarro-Cerrillo ; Chedly Abdelly ; Jesús Jorrín-NovoSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines végétales, Régulation de l'expression des gènes végétaux.
- microbiologie : Quercus.
- métabolisme : Protéines végétales.
- méthodes : Protéomique.
- physiologie : Phytophthora, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Gene Expression Regulation, Plant.
- chemical , metabolism : Plant Proteins.
- methods : Proteomics.
- microbiology : Quercus.
- physiology : Gene Expression Regulation, Plant, Phytophthora.
Abstract
Phytophthora cinnamomi is one of the agents that trigger the decline syndrome in Quercus spp., this being a serious threat to Mediterranean Holm oak forest sustainability and reforestation programs. Quercus ilex responses to Phytophthora cinnamomi have been studied in one-year olds seedlings from two Andalucía provenances, assessing the physiological water status and photosynthesis-related parameters. Upon inoculation with mycelium a reduction in water content, chlorophyll fluorescence, stomatal conductance and gas exchange was observed along a 90 days post inoculation period in both provenances. The reduction was higher in the most susceptible (SSA) provenance, than in the most tolerant (PCO), being these typical plant responses to drought stress. Leaf protein profiles were analyzed in non-inoculated and inoculated seedlings from the two provenances by using a 2-DE coupled to MS proteomics strategy. Ninety seven proteins changing in abundance in response to the inoculation were successfully identified after MALDI-TOF-TOF analyses. The largest group of variable identified proteins were chloroplasts ones, and they were involved in the photosynthesis, Calvin cycle and carbohydrate metabolism. It was noted that a general tendency was a decrease in the protein abundance as a consequence of the inoculation, being it less accused in the least susceptible, the Northern provenance (PCO), than in the most susceptible, the Southern provenance (SSA). This trend is clearly manifested in photosynthesis, amino acid metabolism and stress/defence proteins. On the contrary, some proteins related to starch biosynthesis, glycolysis and stress related peroxiredoxin showed an increase upon inoculation. These changes in protein abundance were correlated to the estimated physiological parameters and have been frequently observed in plants subjected to drought stress.
DOI: 10.1016/j.plaphy.2013.06.030
PubMed: 23962806
Affiliations:
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Electronic address: sghaierbesma@yahoo.fr.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, 14071 Córdoba, Spain; Laboratoire des Plantes Extrêmophiles (LPE), Centre de Biotechnologie à la Technopole de Borj-Cedria (CBBC), BP 901, Hammam-Lif 2050</wicri:regionArea><wicri:noRegion>Hammam-Lif 2050</wicri:noRegion></affiliation></author><author><name sortKey="Valero Galvan, Jose" sort="Valero Galvan, Jose" uniqKey="Valero Galvan J" first="José" last="Valero-Galvàn">José Valero-Galvàn</name></author><author><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M Cristina" last="Romero-Rodríguez">M Cristina Romero-Rodríguez</name></author><author><name sortKey="Navarro Cerrillo, Rafael Ma" sort="Navarro Cerrillo, Rafael Ma" uniqKey="Navarro Cerrillo R" first="Rafael Ma" last="Navarro-Cerrillo">Rafael Ma Navarro-Cerrillo</name></author><author><name sortKey="Abdelly, Chedly" sort="Abdelly, Chedly" uniqKey="Abdelly C" first="Chedly" last="Abdelly">Chedly Abdelly</name></author><author><name sortKey="Jorrin Novo, Jesus" sort="Jorrin Novo, Jesus" uniqKey="Jorrin Novo J" first="Jesús" last="Jorrín-Novo">Jesús Jorrín-Novo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23962806</idno><idno type="pmid">23962806</idno><idno type="doi">10.1016/j.plaphy.2013.06.030</idno><idno type="wicri:Area/Main/Corpus">001230</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001230</idno><idno type="wicri:Area/Main/Curation">001230</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001230</idno><idno type="wicri:Area/Main/Exploration">001230</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Physiological and proteomics analyses of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) responses to Phytophthora cinnamomi.</title><author><name sortKey="Sghaier Hammami, Besma" sort="Sghaier Hammami, Besma" uniqKey="Sghaier Hammami B" first="Besma" last="Sghaier-Hammami">Besma Sghaier-Hammami</name><affiliation wicri:level="1"><nlm:affiliation>Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, 14071 Córdoba, Spain; Laboratoire des Plantes Extrêmophiles (LPE), Centre de Biotechnologie à la Technopole de Borj-Cedria (CBBC), BP 901, Hammam-Lif 2050, Tunisia. Electronic address: sghaierbesma@yahoo.fr.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, 14071 Córdoba, Spain; Laboratoire des Plantes Extrêmophiles (LPE), Centre de Biotechnologie à la Technopole de Borj-Cedria (CBBC), BP 901, Hammam-Lif 2050</wicri:regionArea><wicri:noRegion>Hammam-Lif 2050</wicri:noRegion></affiliation></author><author><name sortKey="Valero Galvan, Jose" sort="Valero Galvan, Jose" uniqKey="Valero Galvan J" first="José" last="Valero-Galvàn">José Valero-Galvàn</name></author><author><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M Cristina" last="Romero-Rodríguez">M Cristina Romero-Rodríguez</name></author><author><name sortKey="Navarro Cerrillo, Rafael Ma" sort="Navarro Cerrillo, Rafael Ma" uniqKey="Navarro Cerrillo R" first="Rafael Ma" last="Navarro-Cerrillo">Rafael Ma Navarro-Cerrillo</name></author><author><name sortKey="Abdelly, Chedly" sort="Abdelly, Chedly" uniqKey="Abdelly C" first="Chedly" last="Abdelly">Chedly Abdelly</name></author><author><name sortKey="Jorrin Novo, Jesus" sort="Jorrin Novo, Jesus" uniqKey="Jorrin Novo J" first="Jesús" last="Jorrín-Novo">Jesús Jorrín-Novo</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Plant (genetics)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Phytophthora (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Proteomics (methods)</term><term>Quercus (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Phytophthora (physiologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (méthodes)</term><term>Quercus (microbiologie)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Quercus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Quercus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Phytophthora</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora cinnamomi is one of the agents that trigger the decline syndrome in Quercus spp., this being a serious threat to Mediterranean Holm oak forest sustainability and reforestation programs. Quercus ilex responses to Phytophthora cinnamomi have been studied in one-year olds seedlings from two Andalucía provenances, assessing the physiological water status and photosynthesis-related parameters. Upon inoculation with mycelium a reduction in water content, chlorophyll fluorescence, stomatal conductance and gas exchange was observed along a 90 days post inoculation period in both provenances. The reduction was higher in the most susceptible (SSA) provenance, than in the most tolerant (PCO), being these typical plant responses to drought stress. Leaf protein profiles were analyzed in non-inoculated and inoculated seedlings from the two provenances by using a 2-DE coupled to MS proteomics strategy. Ninety seven proteins changing in abundance in response to the inoculation were successfully identified after MALDI-TOF-TOF analyses. The largest group of variable identified proteins were chloroplasts ones, and they were involved in the photosynthesis, Calvin cycle and carbohydrate metabolism. It was noted that a general tendency was a decrease in the protein abundance as a consequence of the inoculation, being it less accused in the least susceptible, the Northern provenance (PCO), than in the most susceptible, the Southern provenance (SSA). This trend is clearly manifested in photosynthesis, amino acid metabolism and stress/defence proteins. On the contrary, some proteins related to starch biosynthesis, glycolysis and stress related peroxiredoxin showed an increase upon inoculation. These changes in protein abundance were correlated to the estimated physiological parameters and have been frequently observed in plants subjected to drought stress. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23962806</PMID><DateCompleted><Year>2014</Year><Month>05</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Physiological and proteomics analyses of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) responses to Phytophthora cinnamomi.</ArticleTitle><Pagination><MedlinePgn>191-202</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2013.06.030</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0981-9428(13)00261-1</ELocationID><Abstract><AbstractText>Phytophthora cinnamomi is one of the agents that trigger the decline syndrome in Quercus spp., this being a serious threat to Mediterranean Holm oak forest sustainability and reforestation programs. Quercus ilex responses to Phytophthora cinnamomi have been studied in one-year olds seedlings from two Andalucía provenances, assessing the physiological water status and photosynthesis-related parameters. Upon inoculation with mycelium a reduction in water content, chlorophyll fluorescence, stomatal conductance and gas exchange was observed along a 90 days post inoculation period in both provenances. The reduction was higher in the most susceptible (SSA) provenance, than in the most tolerant (PCO), being these typical plant responses to drought stress. Leaf protein profiles were analyzed in non-inoculated and inoculated seedlings from the two provenances by using a 2-DE coupled to MS proteomics strategy. Ninety seven proteins changing in abundance in response to the inoculation were successfully identified after MALDI-TOF-TOF analyses. The largest group of variable identified proteins were chloroplasts ones, and they were involved in the photosynthesis, Calvin cycle and carbohydrate metabolism. It was noted that a general tendency was a decrease in the protein abundance as a consequence of the inoculation, being it less accused in the least susceptible, the Northern provenance (PCO), than in the most susceptible, the Southern provenance (SSA). This trend is clearly manifested in photosynthesis, amino acid metabolism and stress/defence proteins. On the contrary, some proteins related to starch biosynthesis, glycolysis and stress related peroxiredoxin showed an increase upon inoculation. These changes in protein abundance were correlated to the estimated physiological parameters and have been frequently observed in plants subjected to drought stress. </AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sghaier-Hammami</LastName><ForeName>Besma</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, 14071 Córdoba, Spain; Laboratoire des Plantes Extrêmophiles (LPE), Centre de Biotechnologie à la Technopole de Borj-Cedria (CBBC), BP 901, Hammam-Lif 2050, Tunisia. Electronic address: sghaierbesma@yahoo.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Valero-Galvàn</LastName><ForeName>José</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Romero-Rodríguez</LastName><ForeName>M Cristina</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Navarro-Cerrillo</LastName><ForeName>Rafael Ma</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Abdelly</LastName><ForeName>Chedly</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Jorrín-Novo</LastName><ForeName>Jesús</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>07</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029963" MajorTopicYN="N">Quercus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Holm oak decline</Keyword><Keyword MajorTopicYN="N">Holm oak proteomics</Keyword><Keyword MajorTopicYN="N">Phytophthora cinnamomi</Keyword><Keyword MajorTopicYN="N">Quercus ilex</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>01</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23962806</ArticleId><ArticleId IdType="pii">S0981-9428(13)00261-1</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2013.06.030</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Abdelly, Chedly" sort="Abdelly, Chedly" uniqKey="Abdelly C" first="Chedly" last="Abdelly">Chedly Abdelly</name><name sortKey="Jorrin Novo, Jesus" sort="Jorrin Novo, Jesus" uniqKey="Jorrin Novo J" first="Jesús" last="Jorrín-Novo">Jesús Jorrín-Novo</name><name sortKey="Navarro Cerrillo, Rafael Ma" sort="Navarro Cerrillo, Rafael Ma" uniqKey="Navarro Cerrillo R" first="Rafael Ma" last="Navarro-Cerrillo">Rafael Ma Navarro-Cerrillo</name><name sortKey="Romero Rodriguez, M Cristina" sort="Romero Rodriguez, M Cristina" uniqKey="Romero Rodriguez M" first="M Cristina" last="Romero-Rodríguez">M Cristina Romero-Rodríguez</name><name sortKey="Valero Galvan, Jose" sort="Valero Galvan, Jose" uniqKey="Valero Galvan J" first="José" last="Valero-Galvàn">José Valero-Galvàn</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Sghaier Hammami, Besma" sort="Sghaier Hammami, Besma" uniqKey="Sghaier Hammami B" first="Besma" last="Sghaier-Hammami">Besma Sghaier-Hammami</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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